Sunday, June 6, 2010

activity of microbes isolated from burr comb


Hilary says this needs to be made more understandable. So...will give it a try. The pictures are cell line NCI-H82, selected because it forms easily visable aggregates in suspension. The purpose is to use H82 as a rapid screen for microbial products isolated from routine testing and study interactions. These pictures demonstrate the inhibition/kill of a fungal filtrate identified using these same cells as a rapid screen. The plates were over grown to more easily demonstrate growth by pH color change as well as the obvious aggrates. The controls are the wells with no filtrate added, overgrown with cell aggrates and obvious pH color change. The plates with this fungal filtrate added did not have any visable growth, pH color change and no H82's were seen microscopically. The "blue" picture is a photomicrograph of a control.

Two six well plates were used, one plate was all controls, the other plate (see picture) had two controls and four wells with duplicate amounts of filtrate. A variety of bacteria have been checked, my area of interest, and some inhibition noted, but this easy to work with fungal isolate was selected to try to hone the technique. Antibiotics are not used in the media so microbial contamination is a constant threat.






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